Terminology Service for NFDI4Health
R18 peptide
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http://www.bioassayontology.org/bao#BAO_0001004
The peptide sequence, PHCVPRDLSWLDLEANMCLP, named R18, was identified as an unphosphorylated peptide that binds to 14-3-3 proteins with high affinity (Kd=80 nM). It has been shown to bind to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides. R18 can inhibit the formation of the 14-3-3-Raf-1 complex, reduce Raf-1 dependent transcriptional activity in cells and block the activation of ExoS by 14-3-3. It binds to all the 14-3-3 isoforms with equal affinities. This peptide is useful for competitive inhibition experiments.