Terminology Service for NFDI4Health

cell culture splitting

Go to external page http://purl.obolibrary.org/obo/OBI_0600037


An active cell culture is typically split when it has grown to confluence in its culture dish. Cell culture splitting of a cell culture sample results in an increase in its passage number, which measures how long a sample has been propagated in vitro, and therefore how many selective or genetic changes it is likely to have undergone.

Term info

Label

cell culture splitting

Synonyms
  • cell culture passaging
definition

The act of taking part of a homogeneous cell culture and creating one or more additional separate cultures of similar qualities. input: cell_culture, output cell_culture min cardinality 2. part of cell culturing

definition source

OBI branch derived

editor preferred label

cell culture splitting

example of usage

The act of taking a cell culture of high density, counting the cells, removing part of the cells, and re-seeding a select number of the cells into new flasks with fresh tissue culture media.

has curation status

http://purl.obolibrary.org/obo/IAO_0000125

imported from

http://purl.obolibrary.org/obo/obi.owl

term editor

PlanAndPlannedProcess Branch