A protein-DNA complex assembled at eukaryotic DNA replication origins immediately prior to the initiation of DNA replication. The preinitiation complex is formed by the assembly of additional proteins onto an existing prereplicative complex. In budding yeast, the additional proteins might include Cdc45p, Sld2p, Sld3p, Dpb11p, DNA polymerases, and others; in fission yeast the GINS complex is present. [ http://www.ncbi.nlm.nih.gov/pubmed/12694535 http://www.ncbi.nlm.nih.gov/pubmed/15194812 http://www.ncbi.nlm.nih.gov/pubmed/17230184 ]