Terminology Service for NFDI4Health
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mass spectrometry assay

Go to external page http://purl.obolibrary.org/obo/OBI_0000470

An assay that identifies the amount and type of material entities present in a sample by fragmenting the sample and measuring the mass-to-charge ratio of the resulting particles.

Term info

Label

mass spectrometry assay

definition source

Philippe Rocca-Serra

editor preferred term

mass spectrometry assay

example of usage

Identification of a proteins cut out from a 2D gel by cleaving it into peptides using trypsin digestion using electrospray ionizatino to ensure the peptides are charged, and accelerating them with an electro magnetic field in which the flight path is determined by the mass / charge ratio of the peptides. Comparing the mass/charge ratio of peptides in the proteins with databases of protein sequences allows to identify which protein gave rise to the peptides.

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

term editor

Philippe Rocca-Serra