Terminology Service for NFDI4Health
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tritiated thymidine incorporation assay

Go to external page http://purl.obolibrary.org/obo/OBI_0000669

A cell proliferation assay in which cells are cultured in the presence of tritiated thymidine which is incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle). The radioactivity of tritiated thymidine in a cell is a proxy for cells that were actively replicating.

Term info

Label

tritiated thymidine incorporation assay

editor preferred term

tritiated thymidine incorporation assay

example of usage

The measurement of T cell proliferation as a response to a viral peptide by culturing T cells stimulated with APCs and peptide in the presence of tritiated thymidine, and using a scintillation counter to detect the radioactivity.

has curation status

http://purl.obolibrary.org/obo/IAO_0000120