Terminology Service for NFDI4Health
northern blot assay
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http://purl.obolibrary.org/obo/OBI_0000860
An assay that determines the presence of gene transcripts by hybridizing labeled RNA or DNA probes against messenger RNAs isolated from tissue or cell cultures, resolved on denaturing gel, transfered by a blotting procedure to a solid support. Detection of hybridization signals is carried out by immunofluorescence or radioactivity measurements using photographic films or digital imaging devices such as Phosphor Imager.
Term info
Label
northern blot assay
alternative term
northern blot
editor preferred term
northern blot assay
example of usage
The level of actin mRNA levels in normal and diseased tiisue was measured using a northern blot assay.
has curation status
http://purl.obolibrary.org/obo/IAO_0000123
term editor
Richard Scheuermann, Philippe Rocca-Serra
Term relations
Subclass of:
- analyte assay
- has_specified_output some measurement datum
- realizes some (
analyte role and
inheres in some ribonucleic acid) and has_specified_input some ribonucleic acid