Terminology Service for NFDI4Health
Southern blot assay
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http://purl.obolibrary.org/obo/OBI_0000892
An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity.
Term info
Label
Southern blot assay
alternative term
Southern blot
editor preferred term
Southern blot assay
example of usage
PMID: 9452032. Germline mutations detected in the von Hippel-Lindau disease tumor suppressor gene by Southern blot and direct genomic DNA sequencing.
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
Marcus Chibucos, Philippe Rocca-Serra
Term relations
Subclass of:
- analyte assay
- realizes some (
analyte role and
inheres in some deoxyribonucleic acid) and has_specified_input some deoxyribonucleic acid - has_specified_output some measurement datum
- has part some detection of specific nucleic acid polymers with complementary probes
- has_specified_output some (is about some site)
- realizes some (
evaluant role and
role of some DNA extract) and has_specified_input some (
DNA extract and
has role some evaluant role) - has part some nucleic acid hybridization