Terminology Service for NFDI4Health
promoter activity detection by reporter gene assay
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http://purl.obolibrary.org/obo/OBI_0000913
An assay in which the activity of a promoter in a cell is monitored by using a reporter gene that was inserted in a genomic location under control of the promoter and whose expression can be easily detected based on qualities or functions of the gene.
Term info
Label
promoter activity detection by reporter gene assay
definition source
IEDB
editor preferred term
promoter activity detection by reporter gene assay
example of usage
A T cell hybridoma in which the beta-galactosidase gene (lacZ) was inserted under the control of the IL-2 promoter, is detected by adding the X-gal substrate which when cleaved by lacZ results in detectable blue color.
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
IEDB
Term relations
Subclass of:
- assay
- has_specified_input some cell
- has_specified_output some (is about some core promoter sequence-specific DNA binding)