reverse transcription polymerase chain reaction assay
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http://purl.obolibrary.org/obo/OBI_0001170
An assay that determines the concentration of RNA in a sample in which an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional or real-time PCR.
Term info
Label
reverse transcription polymerase chain reaction assay
definition source
Penn Group
editor preferred term
reverse transcription polymerase chain reaction assay
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
Chris Stoeckert, Jie Zheng
Term relations
Subclass of:
- polymerase chain reaction assay
- has part some reverse transcribed polymerase chain reaction
- has_specified_output some (is about some gene expression)
- realizes some (
evaluant role and
role of some RNA extract) and has_specified_input some (
RNA extract and
has role some evaluant role) - has_specified_input some RNA extract
- has_specified_output some information content entity