Terminology Service for NFDI4Health
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antibody binding detection by fluorescence quenching

Go to external page http://purl.obolibrary.org/obo/OBI_0001635

A binding assay in which affinity is measured by detecting a change in fluorescence (usually quenching) that occurs upon binding of the antibody to the ligand. The fluorescent signal that is affected by binding is either from an exogenous fluorophore attached to the ligand, or is the intrisic fluorescence of aromatic (tryptophan) residues on the binding site of the antibody (no conjugated fluorophore necessary) or, less commonly, on the ligand binding region (epitope).

Term info

Label

antibody binding detection by fluorescence quenching

editor preferred term

antibody binding detection by fluorescence quenching

example of usage

The binding affinity of Fab 4-4-20 for the fluorescein molecule was determined using the fluorescence quenching assay, by placing the Fab and the fluorescein together in a well and measuring the fluorescence after each addition of increasing concentrations of the Fab (PMID: 8637844).

has curation status

http://purl.obolibrary.org/obo/IAO_0000120