Terminology Service for NFDI4Health
purified MHC competitive binding assay measuring equilibrium dissociation constant [KD] of a MHC:ligand complex approximated by IC50 using fluorescence detection
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http://purl.obolibrary.org/obo/OBI_0001667
A purified MHC ligand binding equilibrium dissociation constant (KD) determination assay that uses fluorescence detection to detect loss of binding of a known reference ligand due to competition by the ligand under investigation and provides IC50 values determined under assay conditions where the IC50 approximates a KD value.
Term info
Label
purified MHC competitive binding assay measuring equilibrium dissociation constant [KD] of a MHC:ligand complex approximated by IC50 using fluorescence detection
IEDB alternative term
dissociation constant KD (~IC50)|purified MHC/competitive/fluorescence|nM
definition source
IEDB
editor preferred term
purified MHC competitive binding assay measuring equilibrium dissociation constant [KD] of a MHC:ligand complex approximated by IC50 using fluorescence detection
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
PERSON:Randi Vita, Jason Greenbaum, Bjoern Peters
Term relations
Subclass of:
- purified MHC competitive binding assay measuring equilibrium dissociation constant [KD] of a MHC:ligand complex using fluorescence detection
- has part some fluorescence detection assay
- has_specified_output some (has measurement unit label value nanomolar)
- has_specified_input some (
purified MHC molecule preparation and
has part some MHC protein complex) - has_specified_input some (
material entity and
has role some competitive binding reference ligand role)