Terminology Service for NFDI4Health
split-ubiquitin assay
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http://purl.obolibrary.org/obo/OBI_0001668
A binding assay that screens membrane soluble proteins by fusion of two integral membrane proteins (bait and prey) to two different ubiquitin moieties. One moiety is also fused to a transcription factor (TF) that can be cleaved off by ubiquitin specific proteases. Upon bait and prey interaction, Nub and Cub-moieties assemble, reconstituting the split-ubiquitin. The reconstituted split-ubiquitin molecule is recognized by ubiquitin specific proteases, which cleave off the reporter protein, allowing it to induce the transcription of reporter genes.
Term info
Label
split-ubiquitin assay
definition source
url:https://en.wikipedia.org/wiki/Two-hybrid_screening
editor preferred term
split-ubiquitin assay
example of usage
Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase. PMID:17513562
has curation status
http://purl.obolibrary.org/obo/IAO_0000122
term editor
Marcus Chibucos, Philippe Rocca-Serra