Terminology Service for NFDI4Health
far-Western blot assay
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http://purl.obolibrary.org/obo/OBI_0001669
An assay that measures protein-protein interactions by separating target proteins on an SDS-PAGE gel, transfering them to a solid substrate, hybridizing with a protein probe and visualizing bound proteins using a probe-directed antibody. This is a adaptation on the western blot assay.
Term info
Label
far-Western blot assay
definition source
PMID:18079728
editor preferred term
far-Western blot assay
example of usage
Studying protein-protein interactions via blot overlay or Far Western blot. PMID:15064457
has curation status
http://purl.obolibrary.org/obo/IAO_0000122
term editor
Marcus Chibucos, Philippe Rocca-Serra
Term relations
Subclass of:
- binding assay
- has_specified_input some protein-containing complex
- has_specified_output some (is about some protein domain specific binding)
- has_specified_output some data item