Terminology Service for NFDI4Health
RNA protection assay
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http://purl.obolibrary.org/obo/OBI_0001670
An assay that determines the presence and estimates abundance of transcript species by first creating an homo or heteroduplex by adding a specific, complementary sequence to the sequence of interest and then exposing the mixture of ribonuclease, which will degrade only single stranded molecules. A detection step will reveal if the sample contained a sequence of interest.
Term info
Label
RNA protection assay
alternative term
RPA, RNAse protection assay
definition source
PMID:16491611
editor preferred term
RNA protection assay
example of usage
Absolute concentrations of mRNA for type I and type VI collagen in the canine meniscus in normal and ACL-deficient knee joints obtained by RNase protection assay. PMID:11518275
has curation status
http://purl.obolibrary.org/obo/IAO_0000122
term editor
Marcus Chibucos, Philippe Rocca-Serra
Term relations
Subclass of:
- assay
- has_specified_output some (is about some gene expression)
- has_specified_input some RNA extract
- has_specified_output some data item