Terminology Service for NFDI4Health
electrophoretic mobility shift assay
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http://purl.obolibrary.org/obo/OBI_0001671
An assay that measures information about Protein-DNA or Protein-RNA interactions using gel electrophoresis and relying on the fact the molecular interactions will cause the heterodimer to be retarded on the gel when compared to controls corresponding to protein extract alone and protein extract + neutral nucleic acid.
Term info
Label
electrophoretic mobility shift assay
alternative term
band shift assay, gel mobility shift assay, gel shift assay, gel retardation assay, EMSA
definition source
PMID:6269071
editor preferred term
electrophoretic mobility shift assay
example of usage
Electrophoretic mobility shift assay reveals a novel recognition sequence for Setaria italica NAC protein. PMID:21918373
has curation status
http://purl.obolibrary.org/obo/IAO_0000122
term editor
Marcus Chibucos, Philippe Rocca-Serra
Term relations
Subclass of:
- binding assay
- has_specified_output some (is about some sequence-specific DNA binding)
- has_specified_input some RNA extract
- has_specified_output some data item