bacterial one-hybrid assay
An assay that identifies the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences, is cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence
Term info
bacterial one-hybrid assay
B1H
url:http://en.wikipedia.org/wiki/Bacterial_one-hybrid_system
bacterial one-hybrid assay
A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. PMID:18332042
http://purl.obolibrary.org/obo/IAO_0000122
Marcus Chibucos, Philippe Rocca-Serra