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RNP (ribonuclear particle) immunoprecipitation high- throughput sequencing assay

http://purl.obolibrary.org/obo/OBI_0001857

An analyte assay that combines immunoprecipitation of an RNA-binding protein and RNA-seq to identify mRNAs associated with selected RNA binding proteins (RBPs).

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Term info

Label

RNP (ribonuclear particle) immunoprecipitation high- throughput sequencing assay

alternative term

RIP-seq, RIP-seq assay

definition source

PMID:21172659

editor preferred term

RNP (ribonuclear particle) immunoprecipitation high- throughput sequencing assay

example of usage

Zhao et al. Genome-wide identification of polycomb-associated RNAs by RIP-seq. Molecular Cell (2010) vol. 40 (6) pp. 939-53 [PMID:21172659]

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

term editor

Venkat Malladi, Chris Stoeckert, Jie Zheng

Term relations

Subclass of:

analyte assay
sequencing assay
achieves_planned_objective some protein and RNA interaction identification objective
has part some library preparation
has_specified_output some DNA sequence data
realizes some (
function and
inheres in some sonicator) and has_specified_input some sonicator
has part some RNA sequencing assay
has part some immunoprecipitation
realizes some (
function and
inheres in some DNA sequencer) and has_specified_input some DNA sequencer
realizes some (
    evaluant role and
    role of some protein-containing complex) and has_specified_input some (
    protein-containing complex and
    has role some evaluant role)
has part some cross linking