Terminology Service for NFDI4Health
cross-linking immunoprecipitation high-throughput sequencing assay
Go to external page
http://purl.obolibrary.org/obo/OBI_0001858
An analyte assay that employs UV-crosslinking between RNA and a protein, followed by immunoprecipitation with antibodies for the protein, fragmentation, and high-throughput screening for RNA sequences that interact with a particular RNA-binding protein.
Term info
Label
cross-linking immunoprecipitation high-throughput sequencing assay
alternative term
HITS-CLIP, CLIP-seq, CLIP-seq assay
definition source
PMID:18978773
editor preferred term
cross-linking immunoprecipitation high-throughput sequencing assay
example of usage
Heulga et al. Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins. Cell Rep. 2012 Feb 23;1(2):167-78. [PMID:22574288]
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
Venkat Malladi, Chris Stoeckert, Jie Zheng
Term relations
Subclass of:
- analyte assay
- sequencing assay
- achieves_planned_objective some protein and RNA interaction identification objective
- has part some library preparation
- has_specified_output some DNA sequence data
- has part some RNA sequencing assay
- has part some immunoprecipitation
- realizes some (
function and
inheres in some DNA sequencer) and has_specified_input some DNA sequencer - realizes some (
evaluant role and
role of some protein-containing complex) and has_specified_input some (
protein-containing complex and
has role some evaluant role) - has part some cross linking