Terminology Service for NFDI4Health
cytometry time of flight assay
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http://purl.obolibrary.org/obo/OBI_0002115
A cytometry assay in which the presence of molecules of interest on or in cells is indicated by binding of antibodies labeled with rare earth element tags which are detected by time-of-flight mass spectrometry.
Term info
Label
cytometry time of flight assay
alternative term
CyTOF
definition source
PMID:26190063
editor preferred term
cytometry time of flight assay
example of usage
Whole human blood samples were incubated with lipopolysaccharide (LPS) to examine dose-dependent signaling responses within the toll-like receptor 4 (TLR4) pathway. After LPS stimulation, rare earth metal tagged antibodies against phenotypic markers were used to stain the blood sample (Nd143-CD45RA, Nd145-CD4, Er170-CD3, Sm152-TCR__, Pr141-CD7, Nd146-CD8, Nd142-CD19). A Fluidigm CyTOF version 1 instrument equipped with CyTOF software version 5.1.648 was used to measure and analyze the levels of antibody staining refelctive of the level of expression of those surface receptors.
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
ImmPort
Term relations
Subclass of:
- cytometry assay
- realizes some (
molecular label role and
inheres in some rare earth metal atom) and has_specified_input some rare earth metal atom - has part some mass spectrometry assay