Terminology Service for NFDI4Health
methidiumpropyl-EDTA-iron(II) footprinting assay
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http://purl.obolibrary.org/obo/OBI_0002443
A footprinting assay that determines protein-binding sites on DNA by partial cleavage of ligand-protected DNA restriction fragments with methidium-propyl-EDTA (MPE). MPE-Fe(II) in the presence of oxygen efficiently catalyzes the non-specific clevage of DNA.
Term info
Label
methidiumpropyl-EDTA-iron(II) footprinting assay
alternative term
MPE-Fe(II) footprinting
definition source
PMID:6435669, PMID:6225070
editor preferred term
methidiumpropyl-EDTA-iron(II) footprinting assay
has curation status
http://purl.obolibrary.org/obo/IAO_0000120
term editor
Jie Zheng
Term relations
Subclass of:
- footprinting assay
- has_specified_output some binding datum
- realizes some (
reagent role and
inheres in some EDTA methidiumpropylamide) and has_specified_input some EDTA methidiumpropylamide - realizes some (
evaluant role and
role of some DNA extract) and has_specified_input some (
DNA extract and
has role some evaluant role)