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transcription start site mapping by primer extension assay

http://purl.obolibrary.org/obo/OBI_0002445

A transcription profiling assay in which the transcription start site for a gene is determined by identifying the 5' end of mRNA. A radio-labeled primer is annealed to a complementary mRNA sequence near the 3' end, then cDNA is synthesized until the 5' end is reached.

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Term info

Label

transcription start site mapping by primer extension assay

definition source

PMID:23378648, url:https://en.wikipedia.org/wiki/Primer_extension

editor preferred term

transcription start site mapping by primer extension assay

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

term editor

Rebecca Jackson

Term relations

Subclass of:

transcription profiling assay
has_specified_output some (is about some gene expression)
achieves_planned_objective some transcription start site identification objective
has_specified_output some sequence data
has_specified_input some RNA extract