Terminology Service for NFDI4Health
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precision nuclear run-on sequencing assay

Go to external page http://purl.obolibrary.org/obo/OBI_0002457

An RNA-seq assay that maps the genome-wide distribution of transcriptionally-engaged Pol II at base-pair resolution by using biotin-labeled ribonucleotide triphosphate analogs (biotin-NTP) for nuclear run-on reactions, allowing the efficient affinity purification of nascent RNAs for high throughput sequencing from their 3' ends.

Term info

Label

precision nuclear run-on sequencing assay

alternative term

PRO-Seq, precision nuclear run-on and sequencing assay

definition source

PMID:23430654

editor preferred term

precision nuclear run-on sequencing assay

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

term editor

Rebecca Jackson

term tracker item

https://github.com/obi-ontology/obi/issues/876