Terminology Service < Semantic Lookup Platform

All terms in BAO

Label	Id	Description
detected entity	BAO_0002798
ELISA	BAO_0000134	[Enzyme linked immunosorbent assay or ELISA is used to detect the presence of a particular protein, either an antigen or antibody in the sample such as a body fluid.]
ex situ immunoassay	BAO_0000466	[Ex situ refers to out of place or position, with respect to the antigen that is being tested in the immunoassay. Usually the antigen is present in a tissue or cell lysate, or as a purified protein in the assay.]
oligonucleotide array	BAO_0000133	[Oligonucleotides are immobilized by spotting on a solid support, such as a glass slide. They are hybridized with labeled nucleic acids from a sample and the signals measured, which reflects the composition of the sample nucleic acids. This is performed to either quantify gene expression or genomic profiling to understand genetic variation in humans.]
nucleic acid array	BAO_0000504	[Nucleic acids, either DNA or RNA are immobilized by spotting on a solid support such as a glass slide. They are hybridized with labeled nucleic acids from a sample and the signals measured, which reflects the composition of the sample nucleic acids. This is performed to quantify gene expression under various situations, e.g. diseases, development, etc.]
competitive immunoassay	BAO_0000132	[A competitive binding assay is based upon the competition of labeled and unlabeled ligand for a limited number of antibody binding sites. Briefly, the free antigen and antibody are incubated to form antigen-antibody complex and then the complex are bound to antigen-coated surface in the assay plate. The unbound antibody-antigen complex is washed off before adding enzyme-linked secondary antibody against the primary antibody. The substrate is then added and the antigen concentration can then be determined by the signal strength elicited by the enzyme-substrate reaction. In this assay, the enzyme-linked secondary antibody compete with the sample antigen which is associated with the primary antibody.]
positively correlated	BAO_0000131
endpoint action correlation	BAO_0000103	[Endpoint action correlation describes weather a low or high endpoint value corresponds to low or high activity. Specifically if the endpoint value is positively or inversely correlated with the action that corresponds to the endpoint and which is specified as 'mode of action'. For example 'percent control' can be positively correlated with enzyme activity (remaining activity) and inversely correlated with the mode of action (function) inhibition depending on how the signal is normalized.]
alphascreen	BAO_0000130	[This technology measures the interaction of two molecules bioconjugated to donor and acceptor beads. Laser excitation leads to light emission which is directly proportional to the amount of binding, provided molecules are in proximity to cause a chemical reaction (interact). Users have modified this and coupled it to chemiluminescence emission. This technology has been adapted to study protein-protein, protein-DNA and receptor-ligand, immunoassay, phosphorylation and protease reactions.]
luminescence method	BAO_0000045	[The emission of light from a substance that occurs from an electronically excited state which is reached either by a physical, mechanical, or chemical mechanism.]
hydrolase activity assay	BAO_0002790
enzyme activity assay	BAO_0002994	[It is used to measure the effect of a perturbagen on the enzyme activity, which can be measured either directly or coupled to a secondary signal. ]
ADP coupled enzyme activity measurement method	BAO_0002791
substrate coupled enzyme activity measurement method	BAO_0002593
oxidoreductase activity assay	BAO_0002792
lyase activity assay	BAO_0002793
direct enzyme activity measurement method	BAO_0000139	[An enzyme is a protein that acts as a catalyst for specific biochemical reaction, converting specific substrates into chemically distinct products. Enzyme activity assays quantify the kinetics of the product formation or substrate depletion in the reaction, which result in fluorescence, luminescence or colorimetric outputs.]
isomerase activity assay	BAO_0002794
molecular structure	BAO_0000138
molecular quality	PATO_0002182	[A quality which inheres in a molecular entity, a single molecule, atom, ion, radical etc.]