Terminology Service < Semantic Lookup Platform

All terms in BAO

Label	Id	Description
dehydrogenase activity determination	BAO_0000166	[An expansion in the number of viable cells results in an increase in the overall activity of mitochondrial dehydrogenases in the sample. The internal environment of the proliferating cell is more reduced than that of non-proliferating cells. Specifically, the ratios of NADPH/NADP, FADH/FAD, FMNH/FMN, and NADH/NAD, increase during proliferation. Compounds such as tetrazolium salts and Alamar Blue, which can be reduced by these metabolic intermediates, can be used to monitor cell proliferation. Their reduction is accompanied by a measurable shift in wavelength with a corresponding change in color.]
caspase activity determination	BAO_0000165	[Caspases are cysteine proteases which play an important role in apoptosis. They are synthesized and stored in cells as inactive procaspases. Once apoptosis is initiated in a cell, the procaspases get activated by proteolytic cleavage. This sets in motion a caspase cascade, which leads to the activation of proteins involved in the chromatin fragmentation and finally culminates in cell death.]
DEVD-NucView 488	BAO_0003050
fluorescence microscopy	BAO_0000450	[Fluorescence microscopy is used to image the distribution of molecules based on the properties of fluorescence emission. Excitation of fluorescent molecules with a specific wavelength results in the production of an image by the secondary fluorescence emission at longer wavelengths.]
ATP quantitation	BAO_0000164	[ATP levels are an indicator of viable cell number. ATP values higher than controls (untreated cells) indicate proliferation and cultures with ATP concentrations lower than controls indicate cytotoxicity. ATP levels can be measured using different commercial kits.]
electrical sensor	BAO_0000163	[Electrical sensors are used to measure the electrical impedance, or the current-voltage relationships of these ion channels.]
label free method	BAO_0000427	[It allows the detection of binding interactions and cell-based reactions without the need to use conventional labels, e.g., fluorescent probes. The advantages include the ability to measure a) functional activity without modifying the binding partners with labels, b) binding interactions independent of functional activity, and c) cell-Based GPCR assays without the need for engineering cell-lines to over-express given receptors.]
hematopoietic system disease	DOID_74	[An immune system disease that has_material_basis_in hematopoietic cells.]
metal ion redistribution assay	BAO_0000162	[Metal ions such as copper, iron, magnesium and zinc are transported between different cellular compartments to bring about specific functions. This is regulated by their influx and efflux across membranes, which could occur either by passive diffusion or active transport.]
redistribution assay	BAO_0002999	[It is performed to measure the redistribution of organelles and molecules within the cell (proteins, second messengers, metal ions, dyes, etc. ). This is visualized by microscopy. ]
circular dichroism	BAO_0000161	[Circular dichroism (CD) is a technology for assessing interactions of chiral molecules with circularly polarized electromagnetic rays. Secondary and tertiary structure of proteins will impart a distinct CD to that molecule allowing the use of CD to examine the protein folding under different conditions. Information about conformational properties of nucleic acids can be obtained by CD spectroscopy.]
cannabis abuse	DOID_9505	[A substance abuse that involves the recurring use of cannabis despite negative consequences.]
substance abuse	DOID_302	[A substance-related disorder that involves a maladaptive pattern of substance use leading to significant impairment in functioning.]
ethmoid sinusitis	DOID_9507	[A sinusitis which involves infection of ethmoid sinuses that causes pain or pressure behind and between the eyes, tearing, and headache over the forehead.]
sinusitis	DOID_0050127	[A paranasal sinus disease involving inflammation of the paranasal sinuses resulting from bacterial, fungal, viral infection, allergic or autoimmune issues. Symptoms can include fever, weakness, fatigue, cough and congestion. There may also be mucus drainage in the back of the throat, called postnasal drip.]
dna content estimation	BAO_0000169	[Cellular nuclei can be by stained with Hoechst, DAPI, etc, which is a measure of cell viability and proliferation.]
Syto 60	BAO_0000047
fluorescence intensity	BAO_0000363	[Fluorescence intensity is the measure of fluorescence from a sample.]
vitamin K antagonist	CHEBI_55347
endpoint	BAO_0000179	[The endpoint is a quantitive or qualitative interpretable standardized representation of a perturbation (a change from a defined reference state of a "closed" model system) that is measured by the bioassay. An endpoint consists of a series of data points, one for each perturbing agent (screened entity) tested the assay.]